Rhodamine B
Chemical compound / From Wikipedia, the free encyclopedia
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Rhodamine B /ˈroʊdəmiːn/ is a chemical compound and a dye. It is often used as a tracer dye within water to determine the rate and direction of flow and transport. Rhodamine dyes fluoresce and can thus be detected easily and inexpensively with fluorometers.
Quick Facts Names, Identifiers ...
Names | |
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Preferred IUPAC name
9-(2-Carboxyphenyl)-6-(diethylamino)-N,N-diethyl-3H-xanthen-3-iminium chloride | |
Other names
Rhodamine 610, C.I. Pigment Violet 1, Basic Violet 10, C.I. 45170 | |
Identifiers | |
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3D model (JSmol) |
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ChEBI | |
ChEMBL | |
ChemSpider |
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ECHA InfoCard | 100.001.259 |
KEGG |
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PubChem CID |
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UNII | |
CompTox Dashboard (EPA) |
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Properties | |
C28H31ClN2O3 | |
Molar mass | 479.02 |
Appearance | red to violet powder |
Melting point | 210 to 211 °C (410 to 412 °F; 483 to 484 K) (Decomposes) |
8 to 15 g/L (20 °C)[1][nt 1] | |
Hazards | |
Safety data sheet (SDS) | MSDS |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Rhodamine B is used in biology as a staining fluorescent dye, sometimes in combination with auramine O, as the auramine-rhodamine stain to demonstrate acid-fast organisms, notably Mycobacterium. Rhodamine dyes are also used extensively in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA.[citation needed]