DAPI
Fluorescent stain / From Wikipedia, the free encyclopedia
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DAPI (pronounced 'DAPPY', /ˈdæpiː/), or 4′,6-diamidino-2-phenylindole, is a fluorescent stain that binds strongly to adenine–thymine-rich regions in DNA. It is used extensively in fluorescence microscopy. As DAPI can pass through an intact cell membrane, it can be used to stain both live and fixed cells, though it passes through the membrane less efficiently in live cells and therefore provides a marker for membrane viability.
Quick Facts Names, Identifiers ...
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IUPAC name
2-(4-Amidinophenyl)-1H-indole-6-carboxamidine | |
Other names
4′,6-Diamidino-2-phenylindole | |
Identifiers | |
3D model (JSmol) |
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ChEBI | |
ChEMBL | |
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PubChem CID |
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UNII | |
CompTox Dashboard (EPA) |
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Properties | |
C16H15N5 | |
Molar mass | 277.331 g·mol−1 |
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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